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Policy#: 529-342

Title: ANIMAL USE PROCEDURE: Required Documentation for the Use of Live, Embryonated Eggs

Effective: 9/19/2016

Link: http://redit.ucr.edu/OrApps/RED/Policies.aspx?k=139

In accordance with NIH/OLAW recommendation, this policy addresses the use of live embryonated eggs.

  1. Egg laying adults: For those projects utilizing egg laying adults, the investigator must maintain an approved AUP covering the adult animals needed to produce the eggs.  The AUP must include PI assurance that eggs will only be shared with other laboratories that have an approved AUP or a registered Use of Live, Embryonated Eggs Form.
  2. Eggs obtained from outside sources (e.g. chicken eggs purchased from a supplier or fish eggs obtained from another laboratory):  a. For those projects where eggs will be destroyed in less than 2/3 of the standard embryonic period, or 3 days post fertilization for zebrafish (alterations of the 2/3 time limit may be adjusted for other species of <6 day embryonic period), the investigator must submit a Use of Live, Embryonated Eggs Form, renewed on a 3 year basis.  b. For those projects where the eggs will be allowed to come closer to term, although hatching may not be planned, the investigator must maintain an approved AUP covering the euthanasia of animals that hatch unexpectedly.
  3. Sharing/distribution of live, embryonated eggs: If eggs are shared or distributed between laboratories, each PI must either submit an AUP or a Use of Live, Embryonated Eggs Form, as appropriate.
  4. Use of Live, Embryonated Eggs Forms must be accepted by the Chair and Campus Veterinarian (IACUC members may be substituted if either or both are unavailable).

Reference
http://grants.nih.gov/grants/olaw/faqs.htm#591
Although avian and other egg-laying vertebrate species develop backbones prior to hatching, OLAW interprets the PHS Policy as applicable to their offspring only after hatching. The egg-laying adult animal is covered by the Policy. OLAW expects Assured institutions to have policies and procedures in place that address the care or euthanasia of animals that hatch unexpectedly.


 

 

UC Riverside Institutional Animal Care and Use Committee (IACUC)

 Use of Live, Embryonated Eggs Form

This form should only be used if eggs will be destroyed within 2/3 of the standard embryonic period (alternative for zebrafish: 3 days post fertilization).  If eggs will be used beyond this time, an Animal Use Protocol is required

 

E-mail to: iacuc@ucr.edu

Please use a minimum font size of 10

Submitted:

Registered:

Expires:

 

1.0

Investigator

Last Name:

 

First:

 

Middle:

 

Email:

 

Department

 

Phone:

 

 

 

Species:

 

Source (if obtained from another laboratory, please provide approved AUP number):

 

Standard embryonic period for this species:

 

Maximum time between fertilization and egg destruction:

 

PI Assurance:

___  Eggs will be destroyed within 2/3 of the embryonic period and are not expected to hatch.

____The IACUC (iacuc@ucr.edu) will be informed if any eggs hatch.

____Eggs will only be shared with laboratories maintaining either an AUP or Use of Live, Embryonated Eggs Form for the embryonic stage of this species.

 

 

Description of Project (1-4 sentences):