I:
Related Policies and Guidance
529-283: Animal Care - UC
Riverside Vivaria Facility and Room Entry Restrictions
529-263: Animal Use
- Tumor Production in Rodents
II: Background
Tumors,
cell lines and other biological materials, including serum and embryonic stem
cells, may be contaminated with adventitious rodent pathogens. Usually this
occurs by deriving cell lines or other biologicals from rodents, or by
passaging them through rodents that are themselves contaminated. Many rodent
pathogens survive quite well in cell cultures and may be passed back into other
rodents. Most of the contaminants are known to affect tumorigenesis, the immune
system or other physiological parameters. One contaminant, Lymphocytic Choriomeningitis
Virus (LCMV), is a known zoonotic.
Inadvertent
introduction of untested biologicals carrying pathogens can endanger the health
of rodents in the campus vivaria. Therefore, all biological materials of
unknown status should be tested prior to incorporation into rodents.
III: Guidance
The
following materials must be tested for pathogens, and the results provided to
the Campus Veterinarian for approval, prior to their use in live animals:
Cell
lines, transplantable tumors, tissues, embryonic stem cells, serum components
of cell culture media, ascites fluid, myelomas, hybridomas, spermatozoa, and
any other biological material that will be inoculated into rodents, unless
certified to be pathogen-free (note: the ATCC does not certify lines as
pathogen-free).
The
murine pathogen PCR testing profile should include the following agents (IMPACT
1, IDEXX, or Mouse/Rat Comprehensive Panel, Charles River Laboratories):
|
· Cytomegalovirus
(MCMV)
· Ectromelia
virus
· Hantaan
virus
· K
virus
· Lactate
dehydrogenase-elevating virus (LDHV)
· Lymphocytic
choriomeningitis virus (LCMV)
· Minute
virus of mice (MVM)
· Mouse
adenovirus (MAD)
· Mouse
hepatitis virus (MHV)
|
· Mouse
parvovirus (MPV)
· Mouse
rotavirus
· Mouse
Thymic Virus (MTV)
· Mycoplasma
spp.
· Polyoma
virus
· Pneumonia
virus of mice (PVM)
· Reovirus
3
· Sendai
virus
· Theiler's
murine encephalomyelitis virus (GDVII)
|
The rat
pathogen PCR testing profile should contain agents specific to rat. Contact the
Campus Veterinarian for more information about testing for rat
pathogens.
Laboratories
offering this testing service (these are listed
for investigator convenience, the UCR Office of the Campus Veterinarian does
not require the tests to be performed by these specific labs, nor do we
specifically endorse their services).
· Charles River Laboratories and IDEXX
Bioanalytics offer PCR test panels that detect the presence of rodent
pathogens in biological materials. Additional information can be
found in the sites below.
· https://www.idexxbioanalytics.com/impact-microbiological-rodent-pathogen-testing-profiles
· https://criver.widen.net/s/rdjslhlnvr/rodent-cell-line-biologics
IV: References
Guide
for the Care and Use of Laboratory Animals, (Latest version)
https://www.idexxbioanalytics.com/impact-microbiological-rodent-pathogen-testing
Approved:
01/07/2009; 07/08/2024